Open Access

Effects of high-intensity interval and moderate-intensity continuous running on antioxidant status and muscular performance in physically inactive adults

1 Centre for Fundamental and Continuing Education, Universiti Malaysia Terengganu (UMT), Terengganu, 21030, Malaysia ROR
2 Department Exercise and Sports Science Programme, School of Health Sciences, Universiti Sains, Malaysia ROR
3 Department Exercise and Sports Science Programme, School of Health Sciences, Universiti Sains, Malaysia ROR
4 Department Exercise and Sports Science Programme, School of Health Sciences, Universiti Sains, Malaysia ROR
5 Department Exercise and Sports Science Programme, School of Health Sciences, Universiti Sains, Malaysia ROR
6 Centre for Fundamental and Continuing Education, Universiti Malaysia Terengganu (UMT), Terengganu, 21030, Malaysia ROR

Abstract

The purpose of the present study was to investigate the effect of different types of exercise intensities on antioxidant status and muscular performance in physically inactive adults. A randomised controlled trial was conducted among 36 physically inactive adults, ages 18 to 35 years old. Participants were randomly assigned to one of the three groups (high-intensity interval training (HIIT), moderate-intensity continuous training (MICT) or control) with 12 participants per group (n=12). Over the 8-week intervention period (3 times per week), participants in the HIIT group performed 2 sets of 30 s of running at 85-95% HRmax with 6-8 repetitions on a motorised treadmill. Participants in the MICT group engaged in 30 min of continuous running on a motorised treadmill at 55-70% HRmax. Blood samples were collected to assess total antioxidant status (TAS). The control group did not participate in any exercise training. Muscle performance was measured by using an isokinetic dynamometer, and the angular velocities for knee flexion and extension were set at 180º.s-1 and 300º.s-1, and blood samples were collected to evaluate antioxidant status before and after the 8-week intervention. No significant changes were observed in total antioxidant status between baseline measurements and post-training assessment (p>0.05), nor were there significant differences between groups. Significant improvements in selected muscular performance parameters were observed particularly in the MICT group, while HIIT also demonstrated increases in knee extension average power. In conclusion, while the training interventions did not significantly alter total antioxidant status, the moderate-intensity continuous training programme effectively improved muscular performance in physically inactive adults. These findings suggest that moderate-intensity exercise may be more effective than short-duration interval training for improving neuromuscular performance in physically inactive adults.

Keywords

How to Cite

Suhaimi, M. Z., Muhamad, A. S., Keong, C. C., Kiew, O. F., Li Yin, M. O., & Musa, R. M. (2026). Effects of high-intensity interval and moderate-intensity continuous running on antioxidant status and muscular performance in physically inactive adults. Journal of ROL Sport Sciences, 8, 1–9. https://doi.org/10.70736/jrolss.2384

References

📄 Bogdanis, G. C., Stavrinou, P., Fatouros, I. G., Philippou, A., Chatzinikolaou, A., Draganidis, D., … et al. (2013). Short-term high-intensity interval exercise training attenuates oxidative stress responses and improves antioxidant status in healthy humans. Food and Chemical Toxicology, 61, 171–177. [Crossref]
📄 Canals-Garzón, C., Guisado-Barrilao, R., Martínez-García, D., Chirosa-Ríos, I. J., Jerez-Mayorga, D., & Guisado-Requena, I. M. (2022). Effect of antioxidant supplementation on markers of oxidative stress and muscle damage after strength exercise: A systematic review. International Journal of Environmental Research and Public Health, 19(3), 1803. [Crossref]
📄 Clemente-Suárez, V. J., Bustamante-Sanchez, Á., Mielgo-Ayuso, J., Martínez-Guardado, I., Martín-Rodríguez, A., & Tornero-Aguilera, J. F. (2023). Antioxidants and sports performance. Nutrients, 15(10), 2371. [Crossref]
📄 Clemente, F. M., González-Fernández, F. T., García-Delgado, G., Silva, R., Silva, A. F., Nobari, H., … et al. (2022). Leg dominance and performance in change of directions tests in young soccer players. Scientific Reports, 12(1), 12900. [Crossref]
📄 de Araujo, G. G., Papoti, M., Dos Reis, I. G. M., de Mello, M. A. R., & Gobatto, C. A. (2016). Short- and long-term effects of high-intensity interval training on hormones, metabolites, antioxidant system, glycogen concentration, and aerobic performance adaptations in rats. Frontiers in Physiology, 7, 505. [Crossref]