The aim of this study is to examine the effect of natural and artificial turf fields on muscle damage. A total of 19 male football players with a mean age of 21.63 years participated in the study. A total of eight blood samples were taken from the athletes trained on grass and synthetic ground, before training, immediately after training, 24 and 72 hours after training, and muscle damage after training was evaluated according to the field floor variable. In the detection of muscle damage, creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and troponin T (TnT) markers were used. Friedman and Wilcoxon tests were used for comparisons of the data obtained from the measurements. All statistical calculations were made using the SPSS 26 package program and the error level was accepted as 0.05. According to the results obtained, significant differences were observed in the LDH, AST, CK and TnT levels in time intervals both on turf and synthetic ground (p<0.05). When the field grounds were compared with each other in time intervals, a significant difference was observed in the values of LDH after 24 hours and for TnT before and 72 hours after training (p<0.05), but no significant difference was observed between other times. In conclusion; the analyzes of the obtained data show that both trainings on turf and synthetic field cause muscle damage, but for LDH, CK and AST, which are indicators of muscle damage, recovery occurs faster after training on synthetic ground than on turf. And also, at the point of recovery for TnT, training on grass ground can be claim to be advantageous than synthetic field.