Investigation of PPAR-Alpha gene polymorphism in female’s soccer players
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https://doi.org/10.70736/jrolss.491Keywords:
Polymorphism, PPAR-Alpha, soccer, womenAbstract
The aim of this study was to investigate the effect of PPAR-Alpha gene polymorphism on female soccer players. 24 female soccer players and 24 female sedentary volunteers aged between 18-26 years participated in the study. For DNA isolation, 2 ml of peripheral blood was collected from the study and control groups in EDTA tubes by a specialized nurse from the right or left forearm anterior venous vein. After informed consent forms were filled out, genotyping was performed at the Black Sea Advanced Technology Research and Application Center (KITAM) Laboratory. The polymorphic region of PPAR-α gene (rs4253778) was identified using PCR-RFLP method. A 266 bc segment in intron 7 of the PPAR-α gene containing the C-T substitution was amplified by PCR. PCR products were obtained by creating the amplification reaction. The obtained products were run on agarose gel and 266 bç fragments were evaluated as GG genotype; 253 bç, 216 bç and 50 bç fragments were evaluated as GC genotype and 232 bç and 21 bç fragments were evaluated as CC genotype and statistical analyzes were performed. As a result of the chi-square analysis, there was no difference between the athlete and sedentary groups (p=0.913). The chi-square analysis revealed no significant difference in the distribution of G and C alleles between athletes (G: 23, C: 8) and sedentary individuals (G: 24, C: 6) (p=0.480). In this study, PPAR-Alpha gene polymorphism data will be an important factor in identifying athletes and determining the predisposition of athletes to which sports branches. It can be used to create a database for similar studies on the distribution of PPAR-Alpha genotypes in Turkiye.
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